Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridium kluyveri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADPH | pH 7, 37°C | Clostridium kluyveri | |
1 | - |
NAD+ | pH 7, 37°C | Clostridium kluyveri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Clostridium kluyveri | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | Clostridium kluyveri | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH | NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster | - |
r | |
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | Clostridium kluyveri DSM 555 | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH | NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium kluyveri | A5N5D2 and A5N5D3 | A5N5D2: NfnA, A5N5D3: NfnB | - |
Clostridium kluyveri DSM 555 | A5N5D2 and A5N5D3 | A5N5D2: NfnA, A5N5D3: NfnB | - |
Purification (Comment) | Organism |
---|---|
- |
Clostridium kluyveri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster | in the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+). In the absence of ferredoxin, NAD+ is only very slowly reduced (less than 1% of the rate observed in the presence of ferredoxin) | Clostridium kluyveri | NADH + H+ + 2 NADP+ + reduced ferredoxin iron-sulfur cluster | - |
r | |
NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster | in the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+). In the absence of ferredoxin, NAD+ is only very slowly reduced (less than 1% of the rate observed in the presence of ferredoxin) | Clostridium kluyveri DSM 555 | NADH + H+ + 2 NADP+ + reduced ferredoxin iron-sulfur cluster | - |
r | |
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH | Clostridium kluyveri | NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster | - |
r | |
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH | Clostridium kluyveri DSM 555 | NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster | - |
r | |
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH. In the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+) | Clostridium kluyveri | NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster | - |
r | |
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH. In the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+) | Clostridium kluyveri DSM 555 | NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Clostridium kluyveri |
Synonyms | Comment | Organism |
---|---|---|
NADH-dependent reduced ferredoxin:NADP+ oxidoreductase | - |
Clostridium kluyveri |
NfnAB | - |
Clostridium kluyveri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium kluyveri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Clostridium kluyveri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | iron-sulfur flavoprotein complex. Subunit NfnB contains FAD | Clostridium kluyveri | |
iron-sulfur centre | iron-sulfur flavoprotein complex, subunit NfnA has a predicted [2Fe2S] binding site, subunit NfnB has two predicted [4Fe4S] binding sites. UV-visible spectrum shows that the NfnAB complex contains up to 10 Fe molecules per heterodimer, which is consistent with the presence of two [4Fe4S] and one [2Fe2S] clusters | Clostridium kluyveri |